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A single WAP domain-containing protein from Litopenaeus vannamei possesses antiproteinase activity against subtilisin and antimicrobial activity against AHPND-inducing Vibrio parahaemolyticus.. annealing at 60 °C for LvSWD or 52 °C for EF-1α for 30 s and extension at 72 °C for 30 s and the final extension step at 72 °C for 10 min.
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The WFIKKN1 protein contains a WAP domain, follistatin domain, immunoglobulin domain, two tandem Kunitz domains, and an NTR domain. This gene encodes a WFIKKN1-related protein which has the same domain organization as the WFIKKN1 protein. The WAP-type, follistatin type, Kunitz-type, and NTR-type protease inhibitory domains may control the action of multiple types of proteases.
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Thereby, we cloned a novel 76 aa protein encoding a microglia/macrophage-restricted whey acidic protein (WAP) termed activated microglia/macrophage WAP domain protein (AMWAP). The gene consists of three exons and is located on mouse chromosome 11 in proximity to a chemokine gene cluster. mRNA expression of AMWAP was detected in microglia from.
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WAP domain-containing proteins are initially characterized as milk proteins that present at the mucosal surface. the expression levels of AjWFDC-like was remarkably increased after V. splendidus challenge for 12 h to 72 h, and the peak value was achieved at 72 h post challenge compared with the blank control group (0 h, Fig. 7 A.
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The theoretical isoelectric point (pI) of the mature LvSWD3 protein is 4.41, which is much lower than those of other shrimp mature SWDs (pI = 7.9-8.3). In the C-terminal region, a single WAP domain with a typical WAP domain signature CX 8 CX 6 CX 5 CX 5 CCX 3 CX 3 C could be predicted [40], which covered most region of the mature peptide.
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10/5/24 - Macon, GA - Macon Amphitheater&. Renowned country music artist Jason Aldean has been confirmed as the newest addition to the 2024 summer concert series at Hayden Homes Amphitheater in Bend, Oregon. The announcement made by Live Nation on Monday highlights that Aldean's "Highway Desperado Tour" will make a stop in Central Oregon on.
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The WFDC2 gene is located on human chromosome 20q12-13.1, a region that includes 14 genes that encode proteins with a WAP-type four-disulfide core (WFDC) domain [14, 17].. 55 °C for 30 s, and 72 °C for 34 s. Samples were assayed in duplicate using the ABI Prism 7500 detection system (Perkin Elmer Applied Biosystems). The relative.
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Crispy chicken, pickles, coleslaw, Nashville sauce on a toasted Brioche bun. fries and drinks sold separate. $9.80. Mr Crabs Sandwich, Fries & Drink. Our take on a crab Rangoon with Pacific Northwest crab, cream cheese, sweet chili sauce, and our hand breaded chicken, fresh shredded lettuce on a toasted hoagie with fries and dipping sauce with.